We provide three assemblies for BLAST search of the A. mexicanum genome. To get search results faster, limit the number of sequences in your query and number of reported alignments (set smaller value for the -num_alignments parameter). When searching oligonucleotides (PCR primers, gRNAs) for off-target alignments, use the following command: word_size 11.
1. The AmexG_v6 database was built from a chromosomal scale assembly reported by et al 2021. This assembly was generated from DNA sequences obtained from a single white (d/d) male axolotl. The assembly also includes the mitochondrial genome.
2. The Amex_PQ.v4 database was built from a chromosomal scale assembly reported by Smith et al 2019.
3. The Amex_female.v1 database was built from an assembly used by Keinath et al 2018 to identify the sex-determining region in the axolotl genome. This assembly was generated from DNA sequences obtained from a single wildtype (D/D) female axolotl.